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BL13C1

Monochromatic Protein Crystallography

Biological Crystallography (BX, MX, PX) is a technique used to determine the 3-dimensional structures of biological molecules such as proteins, viruses and nucleic acids (RNA and DNA) to a resolution higher than ~5 Angstrom. From the studies of this structural information, the detailed mechanism of the biological functions carried out by those macromolecules in living cells and organisms can be elucidated. The x-rays deflected ("scattered") by a single crystal of biological molecules concentrate into sharp intense spots (crystal diffraction pattern), and the structure of the biological molecule can be determined by analysis of the intensities and positions of the diffraction spots.

►Contact Information
 
If you have any technical concerns, please contact us!
 
BL13C1 Spokesperson:
Jean, Yuch-Cheng (ycjean@nsrrc.org.tw, +886-3-578-0281 Ext: 7321)
 
BL13C1 Beamline Manager:
Chiang, Cheng-Hung (jjh@nsrrc.org.tw, +886-3-578-0281 Ext: 7369)
 
 
►Technical Information
 

Operational (general use)

Yes, Since Feb. 21th, 2006.

%Time available (general use)

65%

Experiments

HR-SAD, & Monochromatic

Source

28 Poles, 6 cm Period Length, 3.5 Tesla Superconducting Wiggler

Ring Current(mA)

300 mA

Average Beam Lifetime

Infinite Under NSRRC Top Up Conditions

Energy Range

12.4, 12.7, & 13.6 keV (1 Å, 0.976 Å, & 0.915 Å)

Energy Resolution

8 x 10E(-4)

Spot Size

FWHM: 0.68 mm (H) x 0.15 mm (V)

Optics

Vertically Focusing Mirror, Horizontally Focusing Single Crystal Si(111) Bent Monochromator

Flux

4 x 10E(10)

Flux Detailed

Flux Measured Through a 0.2 mm Round Aperture @ 12.7 keV

Wavelength Min (Å)

0.915

Wavelength Max (Å)

1

Monochromator

Horizontally Focusing Single Crystal Monochromator

Crystal Type

Si(111)

Mirrors

Rh Coated

Mirror Cutoff

15 keV

Special Capabilities

Automated Sample Mounting

Goniometer

Huber Single Axis Goniometer

Pin Length

Manual Sample Mounting: No Restrictions; Automated Sample Mounting: 14 - 18 mm

Restrictions

Omega +/- 720 degree

Xtal-detector Distance

100 - 810 mm

Detector

ADSC Quantum-210 CCD Area Detector

Detector Details

Inter-Frame Total Dead Time: 5s

2theta Range

Vertical Offset + 230 mm, -16 mm, Horizontal Offset +/- 80mm

Cryo Capability

Rigaku/MSC X-Stream2000, ~110 K @ Sample

Biohazard

Contact in Advance

Software

BluIce Control Software, HKL/HKL2000, Strategy, CHOOCH

Hardware

3 2-CPU SGI Octane2s, 1 4-CPU SGI O350 Server, 1 Shared 12-CPU SGI Origin3800 Server, Shared 2.0 TB SGI Fiber Channel Raid System

Backup Devices

USB2.0 / IEEE1394 Portable Disk

 
 
 
 
 
►Station Photos
 
BL13C1 Mono Experimental Station Close View of Sample Surrounding
Crystal Handling Area Experimental Control Area
 
 
►Extension Links
 
External Website: http://bionsrrc.nsrrc.org.tw