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BL01A1

Micro Computed Tomography and MRI
 
Optical microscopy is a widely used tool for monitoring cell dynamic. With suitable staining technique, the protein structure and function can be revealed. However, spatial resolution is limited. Transmission electron microscopy (TEM) provides a superior resolution, however, TEM can only probe very thin specimens (<1 um typically). Besides, the process of dehydration, embedding and preparing ultra thin sections is complicated and time-consuming. Synchrotron hard X-ray microscopy currently can provides a resolution, 60 nm to 2 um, bridges the gap between optical and electron microscopy.
Synchrotron radiation provides X-rays that are much more intense, polarized, and continuous over a wide energy range than conventional sources. Even after the beam has undergone monochromatisation, there are still an adequate number of photons for imaging purposes. It has been virtually applied to the medical field as angiography, computed tomography (CT), radiotherapy, X-ray microscopy and structural biology.
Many techniques such as computed tomography and magnetic resonance imaging (MRI) have been developed for imaging the internal structures of live specimens, but these techniques cannot detect the fine micron sized structures. Present in-vitro studies using micro-CT or MRI with phantoms and specimens such as bone and breast tissues can detect objects several ten microns in size. The spatial resolution in micro-CT or MRI with contrast enhancement makes it possible to detect details as small as 20 um. In BL01A1, an attempt was made to observe the internal structures of live specimens using unmonochromatized synchrotron hard X-rays (white beam) and a simple optical detector system.
The BL01A1 system enables the bio-morphological changes of extra fine internal structures in live objects to be studied at micron resolution, regardless of the specific (absorption and/or phase) contrast mechanisms. Higher resolution imaging is possible if a higher magnification lens is used, although the field of view would be narrowed. So that this system could act as a micro-probe for viewing the fine internal structures of live subjects and in a similar way as with light microscopy, various magnification lenses can visualize the microstructures in very thin tissue slices.
Thus, The BL01A1 system can observe extremely fine micron-size structures in live specimens. This would allow more fundamental studies concerning angiogensis (vessel formation), osteogensis (bone formation), and oncogenesis (tumor development) to be done. Clinical applications to human patients for early and accurate detection of tumors, bone and vascular problems, and abnormal changes in the inherent movement of vital organs might have a substantial impact on human health care. For these objectives, further research on the safety aspects, and on the source and detection of X-rays, extending the present limited medical applications of synchrotron radiation are recommended.
  
 
►Contact Information
 
If you have any technical concerns, please contact us!
 
BL01A1 Spokesperson:
Hwu, Yeu-Kuang (phhwu@sinica.edu.tw, +886-3-578-0281 Ext: 3268)
 
BL01A1 Beamline Manager:
Chen, Hsiang-Hsin (hhchen0314@gate.sinica.edu.tw, +886-3-578-0281 Ext: 3274)
 
 
►Technical Information
 

Source

SWLS

Acceptance Angle (mrad)

1 (H) x 0.5 (V)

Energy (keV)

≥ 4

Spot Size (mm)

18.6 (H) x 9.3 (V)

Flux (photons/sec)

1 x 1012

 
 
 
 
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